PDBe 2n3v

Solution NMR

Solution structure of the Rpn1 T1 site with K48-linked diubiquitin in the extended binding mode

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ubiquitin Chains: B, C
Molecule details ›
Chains: B, C
Length: 76 amino acids
Theoretical weight: 8.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P62987 (Residues: 1-76; Coverage: 59%)
Gene names: UBA52, UBCEP2
Sequence domains: Ubiquitin family
26S proteasome regulatory subunit RPN1 Chain: A
Molecule details ›
Chain: A
Length: 131 amino acids
Theoretical weight: 13.64 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P38764 (Residues: 482-612; Coverage: 13%)
Gene names: HRD2, NAS1, RPD1, RPN1, YHR027C
Sequence domains: Proteasome/cyclosome repeat

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 24%
Refinement method: simulated annealing
Expression system: Escherichia coli