PDBe 2n1t

Solution NMR

Dynamic binding mode of a synaptotagmin-1-SNARE complex in solution

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Vesicle-associated membrane protein 2 Chain: A
Molecule details ›
Chain: A
Length: 69 amino acids
Theoretical weight: 8.05 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P63045 (Residues: 25-93; Coverage: 60%)
Gene names: Syb2, Vamp2
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Syntaxin-1A Chain: B
Molecule details ›
Chain: B
Length: 72 amino acids
Theoretical weight: 8.43 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P32851 (Residues: 188-259; Coverage: 25%)
Gene names: Sap, Stx1a
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Synaptosomal-associated protein 25 Chain: C
Molecule details ›
Chain: C
Length: 77 amino acids
Theoretical weight: 9.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P60880 (Residues: 7-83; Coverage: 37%)
  • Best match: P60880-2 (Residues: 7-83)
Gene names: SNAP, SNAP25
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Synaptosomal-associated protein 25 Chain: D
Molecule details ›
Chain: D
Length: 74 amino acids
Theoretical weight: 8.46 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P60880 (Residues: 131-204; Coverage: 36%)
Gene names: SNAP, SNAP25
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Synaptotagmin-1 Chain: E
Molecule details ›
Chain: E
Length: 156 amino acids
Theoretical weight: 17.47 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P21579 (Residues: 272-419; Coverage: 35%)
Gene names: SVP65, SYT, SYT1
Sequence domains: C2 domain
Structure domains: C2 domain

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 13%
Refinement method: molecular dynamics
Chemical shifts: BMR6235  
Expression system: Escherichia coli