PDBe 2mws

Solution NMR

Structure of the complex of ubiquitin and the ubiquitin-like (UBL) domain of Ddi1

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ubiquitin Chain: A
Molecule details ›
Chain: A
Length: 76 amino acids
Theoretical weight: 8.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CG48 (Residues: 609-684; Coverage: 11%)
Gene name: UBC
Sequence domains: Ubiquitin family
DNA damage-inducible protein 1 Chain: B
Molecule details ›
Chain: B
Length: 94 amino acids
Theoretical weight: 10.59 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P40087 (Residues: 2-80; Coverage: 19%)
Gene names: DDI1, VSM1, YER143W
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 47%
Refinement method: simulated annealing
Chemical shifts: BMR25088  
Expression system: Escherichia coli