PDBe 2mtp

Solution NMR

The structure of Filamin repeat 21 bound to integrin

Released:
Source organism: Homo sapiens
Primary publication:
Structural mechanism of integrin inactivation by filamin.
OpenAccess logo Nat. Struct. Mol. Biol. 22 383-9 (2015)
PMID: 25849143

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Filamin-A Chain: A
Molecule details ›
Chain: A
Length: 95 amino acids
Theoretical weight: 9.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P21333 (Residues: 2236-2330; Coverage: 4%)
Gene names: FLN, FLN1, FLNA
Structure domains: Immunoglobulins
Integrin alpha-IIb light chain, form 2 Chain: B
Molecule details ›
Chain: B
Length: 21 amino acids
Theoretical weight: 2.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P08514 (Residues: 1019-1039; Coverage: 2%)
Gene names: GP2B, ITGA2B, ITGAB
Sequence domains: Integrin alpha cytoplasmic region
Integrin beta-3 Chain: C
Molecule details ›
Chain: C
Length: 47 amino acids
Theoretical weight: 5.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P05106 (Residues: 742-788; Coverage: 6%)
Gene names: GP3A, ITGB3
Sequence domains: Integrin beta cytoplasmic domain

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 69%
Refinement method: simulated annealing
Chemical shifts: BMR25176  
Expression system: Escherichia coli