PDBe 2mg5

Solution NMR

Solution Structure of Calmodulin bound to the target peptide of Endothelial Nitrogen Oxide Synthase phosphorylated at Thr495

Released:

Function and Biology Details

Reaction catalysed:
(1a) 2 L-arginine + 2 NADPH + 2 O(2) = 2 N(omega)-hydroxy-L-arginine + 2 NADP(+) + 2 H(2)O
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Calmodulin-2 Chain: A
Molecule details ›
Chain: A
Length: 148 amino acids
Theoretical weight: 16.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DP24 (Residues: 2-149; Coverage: 99%)
Gene names: CALM2, CAM2, CAMB
Sequence domains: EF-hand domain pair
Nitric oxide synthase, endothelial Chain: B
Molecule details ›
Chain: B
Length: 16 amino acids
Theoretical weight: 1.79 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P29474 (Residues: 495-510; Coverage: 1%)
Gene name: NOS3

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 69%
Refinement method: simulated annealing
Chemical shifts: BMR19586  
Expression systems:
  • Escherichia coli
  • Not provided