PDBe 2me6

Solution NMR

NMR Structure of the homeodomain transcription factor Gbx1 from Homo sapiens in complex with the DNA sequence CGACTAATTAGTCG

Source organism: Homo sapiens
Entry authors: Proudfoot A, Serrano P, Geralt M, Wuthrich K, Joint Center for Structural Genomics (JCSG), Partnership for Stem Cell Biology (STEMCELL)

Function and Biology Details

Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Homeobox protein GBX-1 Chain: A
Molecule details ›
Chain: A
Length: 71 amino acids
Theoretical weight: 8.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: Q14549 (Residues: 256-325; Coverage: 19%)
Gene name: GBX1
Sequence domains: Homeodomain
Structure domains: Homeodomain-like
DNA (5'-D(*CP*GP*AP*CP*TP*AP*AP*TP*TP*AP*GP*TP*CP*G)-3') Chains: B, C
Molecule details ›
Chains: B, C
Length: 14 nucleotides
Theoretical weight: 4.28 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 59%
Refinement method: molecular dynamics
Expression systems:
  • Escherichia coli
  • Not provided