PDBe 2mbb

Solution NMR

Solution Structure of the human Polymerase iota UBM1-Ubiquitin Complex

Released:

Function and Biology Details

Reaction catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
DNA polymerase iota; Immunoglobulin G-binding protein G Chain: A
Molecule details ›
Chain: A
Length: 106 amino acids
Theoretical weight: 11.99 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UNA4 (Residues: 516-555; Coverage: 5%)
  • Canonical: P06654 (Residues: 229-282; Coverage: 13%)
  • nullnull
Gene names: POLI, RAD30B, spg
Ubiquitin Chain: B
Molecule details ›
Chain: B
Length: 78 amino acids
Theoretical weight: 8.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CG47 (Residues: 153-228; Coverage: 33%)
Gene name: UBB
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 84%
Refinement method: torsion angle dynamics
Chemical shifts: BMR19394  
Expression system: Escherichia coli