PDBe 2m86

Solution NMR

Solution structure of Hdm2 with engineered cyclotide


Function and Biology Details

Reaction catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
MCo-PMI Chain: A
Molecule details ›
Chain: A
Length: 51 amino acids
Theoretical weight: 5.29 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
E3 ubiquitin-protein ligase Mdm2 Chain: B
Molecule details ›
Chain: B
Length: 129 amino acids
Theoretical weight: 14.71 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: Q00987 (Residues: 17-125; Coverage: 22%)
  • Best match: Q00987-8 (Residues: 1-64)
Gene name: MDM2
Sequence domains: SWIB/MDM2 domain
Structure domains: SWIB/MDM2 domain

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 47%
Refinement method: DGSA-distance geometry simulated annealing
Chemical shifts: BMR19230  
Expression system: Escherichia coli