PDBe 2ly4

Solution NMR

HMGB1-facilitated p53 DNA binding occurs via HMG-box/p53 transactivation domain interaction and is regulated by the acidic tail

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
High mobility group protein B1 Chain: A
Molecule details ›
Chain: A
Length: 83 amino acids
Theoretical weight: 9.71 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P09429 (Residues: 2-84; Coverage: 39%)
Gene names: HMG1, HMGB1
Sequence domains: HMG-box domain
Structure domains: High mobility group box domain
Cellular tumor antigen p53 Chain: B
Molecule details ›
Chain: B
Length: 93 amino acids
Theoretical weight: 9.98 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04637 (Residues: 1-93; Coverage: 24%)
Gene names: P53, TP53
Sequence domains:

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 92%
Refinement method: DGSA-distance geometry simulated annealing
Chemical shifts: BMR18709  
Expression system: Escherichia coli