PDBe 2las

Solution NMR

Molecular Determinants of Paralogue-Specific SUMO-SIM Recognition

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Small ubiquitin-related modifier 1 Chain: A
Molecule details ›
Chain: A
Length: 101 amino acids
Theoretical weight: 11.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: NEW P63165 (Residues: 1-101; Coverage: 100%)
Gene names: OK/SW-cl.43, SMT3C, SMT3H3, SUMO1, UBL1
Sequence domains: Ubiquitin-2 like Rad60 SUMO-like
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1
E3 SUMO-protein ligase RanBP2 Chain: B
Molecule details ›
Chain: B
Length: 13 amino acids
Theoretical weight: 1.62 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: NEW P49792 (Residues: 2705-2717; Coverage: 0%)
Gene names: NUP358, RANBP2

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 31%
Refinement method: simulated annealing
Chemical shifts: BMR17536  
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided