PDBe 2khw

Solution NMR

Solution Structure of the human Polymerase iota UBM2-Ubiquitin Complex

Released:
Entry authors: Bomar MG, D'Souza S, Bienko M, Dikic I, Walker G

Function and Biology Details

Reaction catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
DNA polymerase iota; Immunoglobulin G-binding protein G Chain: A
Molecule details ›
Chain: A
Length: 108 amino acids
Theoretical weight: 12.35 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UNA4 (Residues: 699-740; Coverage: 6%)
  • Canonical: P19909 (Residues: 304-359; Coverage: 10%)
  • nullnull
Gene names: POLI, RAD30B, spg
Ubiquitin Chain: B
Molecule details ›
Chain: B
Length: 79 amino acids
Theoretical weight: 8.86 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CG47 (Residues: 153-228; Coverage: 33%)
Gene name: UBB
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression system: Escherichia coli