PDBe 2kf2

Solution NMR

Solution NMR structure of of Streptomyces coelicolor polyketide cyclase SCO5315. Northeast Structural Genomics Consortium target RR365

Released:
Entry authors: Cort JR, Ramelot TA, Ding K, Wang H, Jiang M, Maglaqui M, Xiao R, Nair R, Baran MC, Everett JK, Swapna GVT, Acton TB, Rost B, Montelione GT, Kennedy MA, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative polyketide cyclase Chain: A
Molecule details ›
Chain: A
Length: 167 amino acids
Theoretical weight: 19.27 KDa
Source organism: Streptomyces coelicolor A3(2)
Expression system: Escherichia coli
UniProt:
  • Canonical: P23154 (Residues: 1-159; Coverage: 100%)
Gene names: SC6G9.18, SCO5315
Sequence domains: Polyketide cyclase / dehydrase and lipid transport
Structure domains: Alpha-D-Glucose-1,6-Bisphosphate; Chain A, domain 4

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 87%
Refinement method: molecular dynamics, simulated annealing
Chemical shifts: BMR16881  
Expression system: Escherichia coli