PDBe 2k4d

Solution NMR

E2-c-Cbl recognition is necessary but not sufficient for ubiquitination activity

Released:

Function and Biology Details

Reaction catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)- ubiquitinyl-[acceptor protein]-L-lysine. 
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase CBL Chain: A
Molecule details ›
Chain: A
Length: 83 amino acids
Theoretical weight: 9.39 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P22681 (Residues: 358-437; Coverage: 9%)
Gene names: CBL, CBL2, RNF55
Sequence domains: Prokaryotic RING finger family 4

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 94%
Refinement method: simulated annealing
Chemical shifts: BMR15796  
Expression system: Escherichia coli