PDBe 2jri

Solution NMR

Solution structure of the Josephin domain of Ataxin-3 in complex with ubiquitin molecule.

Released:
Source organism: Homo sapiens
Entry authors: Nicastro G, Masino L, Esposito V, Menon R, Pastore A

Function and Biology Details

Reaction catalysed:
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ataxin-3 Chain: A
Molecule details ›
Chain: A
Length: 182 amino acids
Theoretical weight: 21.05 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P54252 (Residues: 1-182; Coverage: 50%)
Gene names: ATX3, ATXN3, MJD, MJD1, SCA3
Sequence domains: Josephin
Structure domains:
Ubiquitin Chains: B, C
Molecule details ›
Chains: B, C
Length: 76 amino acids
Theoretical weight: 8.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0CG48 (Residues: 609-684; Coverage: 11%)
Gene name: UBC
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: torsion angle dynamics
Expression system: Escherichia coli BL21(DE3)