PDBe 2jo6

Solution NMR

NMR structure of the E.coli protein NirD, Northeast Structural Genomics target ET100

Released:
Source organism: Escherichia coli
Entry authors: Ramelot TA, Cort JR, Yee AA, Guido V, Lukin JA, Arrowsmith CH, Kennedy MA, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Reaction catalysed:
Ammonia + 3 NAD(+) + 2 H(2)O = nitrite + 3 NADH
Biochemical function:
Biological process:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitrite reductase (NADH) small subunit Chain: A
Molecule details ›
Chain: A
Length: 113 amino acids
Theoretical weight: 12.77 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A9I8 (Residues: 1-108; Coverage: 100%)
Gene names: JW3329, b3366, nirD
Sequence domains: Rieske-like [2Fe-2S] domain
Structure domains: Rieske [2Fe-2S] iron-sulphur domain

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 92%
Refinement method: simulated annealing
Chemical shifts: BMR15139  
Expression system: Escherichia coli