PDBe 2jgu

X-ray diffraction
2.6Å resolution

crystal structure of DNA-directed DNA polymerase

Released:
Source organism: Pyrococcus furiosus
Primary publication:
Crystal structure of Pfu, the high fidelity DNA polymerase from Pyrococcus furiosus.
Int. J. Biol. Macromol. 42 356-61 (2008)
PMID: 18355915

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA polymerase Chain: A
Molecule details ›
Chain: A
Length: 775 amino acids
Theoretical weight: 90.26 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P61875 (Residues: 1-775; Coverage: 100%)
Gene names: PF0212, pol
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 4A
Spacegroup: C2
Unit cell:
a: 92.178Å b: 127.923Å c: 89.21Å
α: 90° β: 109.11° γ: 90°
R-values:
R R work R free
0.275 0.275 0.271
Expression system: Escherichia coli BL21