PDBe 2jgd

X-ray diffraction
2.6Å resolution

E. COLI 2-oxoglutarate dehydrogenase (E1o)

Released:

Function and Biology Details

Reaction catalysed:
2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine = [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO(2)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
2-oxoglutarate dehydrogenase E1 component Chain: A
Molecule details ›
Chain: A
Length: 933 amino acids
Theoretical weight: 105.17 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AFG3 (Residues: 1-933; Coverage: 100%)
Gene names: JW0715, b0726, sucA
Sequence domains:
Structure domains:
2-oxoglutarate dehydrogenase E1 component Chain: B
Molecule details ›
Chain: B
Length: 933 amino acids
Theoretical weight: 105.21 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AFG3 (Residues: 1-933; Coverage: 100%)
Gene names: JW0715, b0726, sucA
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P41212
Unit cell:
a: 142.493Å b: 142.493Å c: 251.811Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.186 0.25
Expression system: Escherichia coli BL21(DE3)