PDBe 2jfc

X-ray diffraction
2.4Å resolution

M144L mutant of Nitrite Reductase from Alcaligenes xylosoxidans in space group P212121

Released:

Function and Biology Details

Reaction catalysed:
Nitric oxide + H(2)O + ferricytochrome c = nitrite + ferrocytochrome c + 2 H(+)
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Copper-containing nitrite reductase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 335 amino acids
Theoretical weight: 36.42 KDa
Source organism: Achromobacter xylosoxidans
Expression system: Escherichia coli
UniProt:
  • Canonical: O68601 (Residues: 26-360; Coverage: 100%)
Gene names: ERS451415_02178, nir, nirK
Sequence domains:
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.1
Spacegroup: P212121
Unit cell:
a: 171.958Å b: 175.91Å c: 181.056Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.171 0.193
Expression system: Escherichia coli