PDBe 2jf4

X-ray diffraction
2.2Å resolution

Family 37 trehalase from Escherichia coli in complex with validoxylamine

Released:

Function and Biology Details

Reaction catalysed:
Alpha,alpha-trehalose + H(2)O = beta-D-glucose + alpha-D-glucose
Biochemical function:
Biological process:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Periplasmic trehalase Chain: A
Molecule details ›
Chain: A
Length: 535 amino acids
Theoretical weight: 61.18 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P13482 (Residues: 31-565; Coverage: 100%)
Gene names: JW1186, b1197, osmA, treA
Sequence domains: Trehalase
Structure domains: Glycosyltransferase

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P212121
Unit cell:
a: 49.399Å b: 102.696Å c: 103.174Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.163 0.214
Expression system: Escherichia coli BL21