PDBe 2j8u

X-ray diffraction
2.88Å resolution

Large CDR3a loop alteration as a function of MHC mutation.

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
HLA class I histocompatibility antigen, A-2 alpha chain Chains: A, H
Molecule details ›
Chains: A, H
Length: 275 amino acids
Theoretical weight: 31.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01892 (Residues: 25-299; Coverage: 81%)
Gene names: HLA-A, HLAA
Sequence domains:
Structure domains:
Beta-2-microglobulin Chains: B, I
Molecule details ›
Chains: B, I
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
ER membrane protein complex subunit 7 Chains: C, J
Molecule details ›
Chains: C, J
Length: 9 amino acids
Theoretical weight: 1.05 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q9NPA0 (Residues: 4-12; Coverage: 0%)
Gene names: C11orf3, C15orf24, EMC7, HT022, UNQ905/PRO1926
T-cell receptor alpha chain C region Chains: E, L
Molecule details ›
Chains: E, L
Length: 194 amino acids
Theoretical weight: 21.66 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P01849 (Residues: 1-14, 15-81; Coverage: 59%)
Gene name: Tcra
Structure domains: Immunoglobulins
T-cell receptor beta chain V region C5 Chains: F, M
Molecule details ›
Chains: F, M
Length: 238 amino acids
Theoretical weight: 26.89 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P04213 (Residues: 8-122; Coverage: 98%)
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 93.422Å b: 83.893Å c: 122.273Å
α: 90° β: 92.21° γ: 90°
R-values:
R R work R free
0.269 0.268 0.293
Expression systems:
  • Escherichia coli
  • Escherichia coli BL21
  • Not provided