PDBe 2j7q

X-ray diffraction
1.8Å resolution

Crystal structure of the ubiquitin-specific protease encoded by murine cytomegalovirus tegument protein M48 in complex with a ubquitin-based suicide substrate

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
MCMV TEGUMENT PROTEIN M48 ENCODED UBIQUITIN- SPECIFIC PROTEASE, M48USP Chain: A
Molecule details ›
Chain: A
Length: 232 amino acids
Theoretical weight: 25.68 KDa
Source organism: Murid betaherpesvirus 1
Expression system: Escherichia coli BL21(DE3)
Ubiquitin Chains: B, D
Molecule details ›
Chains: B, D
Length: 75 amino acids
Theoretical weight: 8.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0CG48 (Residues: 609-683; Coverage: 11%)
Gene name: UBC
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1
MCMV TEGUMENT PROTEIN M48 ENCODED UBIQUITIN- SPECIFIC PROTEASE, M48USP Chain: C
Molecule details ›
Chain: C
Length: 232 amino acids
Theoretical weight: 25.69 KDa
Source organism: Murid betaherpesvirus 1
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments


1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P1
Unit cell:
a: 40.907Å b: 57.298Å c: 67.279Å
α: 73.37° β: 85.37° γ: 88.54°
R-values:
R R work R free
0.157 0.156 0.214
Expression system: Escherichia coli BL21(DE3)