PDBe 2j6e

X-ray diffraction
3Å resolution

Crystal Structure of an Autoimmune Complex between a Human IgM Rheumatoid Factor and IgG1 Fc reveals a Novel Fc Epitope and Evidence for Affinity Maturation

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
IGM Chains: H, I
Molecule details ›
Chains: H, I
Length: 231 amino acids
Theoretical weight: 24.83 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
IGM Chains: L, M
Molecule details ›
Chains: L, M
Length: 234 amino acids
Theoretical weight: 24.76 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
Immunoglobulin heavy constant gamma 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 232 amino acids
Theoretical weight: 26.14 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P01857 (Residues: 99-330; Coverage: 70%)
Gene name: IGHG1
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins

Ligands and Environments

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: C2
Unit cell:
a: 241.98Å b: 75.61Å c: 102.4Å
α: 90° β: 91.13° γ: 90°
R-values:
R R work R free
0.223 0.223 0.288
Expression systems:
  • Homo sapiens
  • Cricetulus griseus