PDBe 2j0s

X-ray diffraction
2.21Å resolution

The crystal structure of the Exon Junction Complex at 2.2 A resolution

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
4 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (5 distinct):
Eukaryotic initiation factor 4A-III Chain: A
Molecule details ›
Chain: A
Length: 410 amino acids
Theoretical weight: 46.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P38919 (Residues: 2-411; Coverage: 100%)
Gene names: DDX48, EIF4A3, KIAA0111
Sequence domains:
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Protein mago nashi homolog Chain: C
Molecule details ›
Chain: C
Length: 146 amino acids
Theoretical weight: 17.19 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61326 (Residues: 1-146; Coverage: 100%)
Gene names: MAGOH, MAGOHA
Sequence domains: Mago nashi protein
Structure domains: Mago nashi
RNA-binding protein 8A Chain: D
Molecule details ›
Chain: D
Length: 89 amino acids
Theoretical weight: 10.22 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y5S9 (Residues: 66-154; Coverage: 51%)
Gene names: HSPC114, MDS014, RBM8, RBM8A
Sequence domains: RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain)
Structure domains: Alpha-Beta Plaits
Protein CASC3 Chain: T
Molecule details ›
Chain: T
Length: 150 amino acids
Theoretical weight: 17.71 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O15234 (Residues: 137-286; Coverage: 21%)
Gene names: CASC3, MLN51
Sequence domains: CASC3/Barentsz eIF4AIII binding
5'-R(*UP*UP*UP*UP*UP*UP*UP*UP*UP*UP *UP*UP*UP*UP*U)-3' Chain: E
Molecule details ›
Chain: E
Length: 15 nucleotides
Theoretical weight: 4.55 KDa

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P64
Unit cell:
a: 169.44Å b: 169.44Å c: 71.04Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.187 0.185 0.216
Expression system: Escherichia coli BL21(DE3)