› 2iwb

X-ray diffraction
1.8Å resolution

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140899 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Methicillin resistance mecR1 protein Chain: A
Molecule details ›
Chain: A
Length: 246 amino acids
Theoretical weight: 29.25 KDa
Source organism: Staphylococcus aureus subsp. aureus N315
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A0B0 (Residues: 340-585; Coverage: 42%)
Gene names: SA0039, mecR, mecR1
Sequence domains: Penicillin binding protein transpeptidase domain
Structure domains: DD-peptidase/beta-lactamase superfamily
TETRAPEPTIDE Chain: B
Molecule details ›
Chain: B
Length: 4 amino acids
Theoretical weight: 431 Da
Source organism: synthetic construct
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

2 bound ligands:
Ligand NI 1 x NI
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM16
Spacegroup: P21212
Unit cell:
a: 57.56Å b: 80.79Å c: 58.88Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.162 0.16 0.203
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Cell envelope stress response in Gram-positive bacteria.
Jordan et al. (2008)
4 more

1 mention without citation

Structural analysis of avibactam-mediated activation of the bla and mec divergons in methicillin-resistant Staphylococcus aureus.
Alexander et al. (2020)