PDBe 2ie4

X-ray diffraction
2.6Å resolution

Structure of the Protein Phosphatase 2A Core Enzyme Bound to okadaic acid

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform Chain: A
Molecule details ›
Chain: A
Length: 589 amino acids
Theoretical weight: 65.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P30153 (Residues: 1-589; Coverage: 100%)
Gene name: PPP2R1A
Sequence domains: HEAT repeat
Structure domains: Leucine-rich Repeat Variant
Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform Chain: C
Molecule details ›
Chain: C
Length: 309 amino acids
Theoretical weight: 35.64 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P67775 (Residues: 1-309; Coverage: 100%)
Gene name: PPP2CA
Sequence domains: Calcineurin-like phosphoesterase
Structure domains: Purple Acid Phosphatase; chain A, domain 2

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: I222
Unit cell:
a: 92.54Å b: 194.85Å c: 201.35Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.25 0.27 0.23
Expression systems:
  • Escherichia coli BL21(DE3)
  • Spodoptera frugiperda