PDBe 2ibw

X-ray diffraction
1.9Å resolution

Crystallographic and kinetic studies of human mitochondrial acetoacetyl-CoA thiolase (T2): the importance of potassium and chloride for its structure and function


Function and Biology Details

Reaction catalysed:
2 acetyl-CoA = CoA + acetoacetyl-CoA
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Acetyl-CoA acetyltransferase, mitochondrial Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 395 amino acids
Theoretical weight: 41.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: P24752 (Residues: 33-427; Coverage: 93%)
Gene names: ACAT, ACAT1, MAT
Sequence domains:
Structure domains: Peroxisomal Thiolase; Chain A, domain 1

Ligands and Environments

Cofactor: Ligand COA 4 x COA

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I711
Spacegroup: P21
Unit cell:
a: 76.143Å b: 107.577Å c: 101.954Å
α: 90° β: 102.89° γ: 90°
R R work R free
0.155 0.153 0.194
Expression system: Escherichia coli