PDBe 2iae

X-ray diffraction
3.5Å resolution

Crystal structure of a protein phosphatase 2A (PP2A) holoenzyme.

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform Chains: A, D
Molecule details ›
Chains: A, D
Length: 589 amino acids
Theoretical weight: 65.39 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q76MZ3 (Residues: 1-589; Coverage: 100%)
Gene name: Ppp2r1a
Sequence domains:
Structure domains: Leucine-rich Repeat Variant
Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit gamma isoform Chains: B, E
Molecule details ›
Chains: B, E
Length: 407 amino acids
Theoretical weight: 48.19 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13362 (Residues: 30-436; Coverage: 78%)
Gene names: KIAA0044, PPP2R5C
Structure domains: Leucine-rich Repeat Variant
Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform Chains: C, F
Molecule details ›
Chains: C, F
Length: 309 amino acids
Theoretical weight: 35.65 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P67775 (Residues: 1-309; Coverage: 100%)
Gene name: PPP2CA
Sequence domains: Calcineurin-like phosphoesterase
Structure domains: Purple Acid Phosphatase; chain A, domain 2
microcystin-LR Chains: M, N
Molecule details ›
Chains: M, N
Length: 7 amino acids
Theoretical weight: 1.01 KDa
Source organism: Microcystis aeruginosa
Expression system: Not provided

Ligands and Environments

1 bound ligand:

4 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P213
Unit cell:
a: 265.301Å b: 265.301Å c: 265.301Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.26 0.257 0.316
Expression systems:
  • Escherichia coli
  • Trichoplusia ni
  • Not provided