PDBe 2i91

X-ray diffraction
2.65Å resolution

60kDa Ro autoantigen in complex with a fragment of misfolded RNA


Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct RNA molecules
Macromolecules (3 distinct):
60 kDa SS-A/Ro ribonucleoprotein Chains: A, B
Molecule details ›
Chains: A, B
Length: 538 amino acids
Theoretical weight: 60.77 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
  • Canonical: P42700 (Residues: 1-538; Coverage: 100%)
Gene name: trove2
Sequence domains: TROVE domain
Structure domains: Rossmann fold
5'-R(*GP*CP*CP*UP*AP*CP*CP*C)-3' Chains: C, E
Molecule details ›
Chains: C, E
Length: 8 nucleotides
Theoretical weight: 2.46 KDa
Source organism: Synthetic construct
Expression system: Not provided
5'-R(*C*GP*GP*UP*AP*GP*GP*CP*UP*UP*UP*UP*CP*AP*A)-3' Chains: D, F
Molecule details ›
Chains: D, F
Length: 15 nucleotides
Theoretical weight: 4.77 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21
Unit cell:
a: 72.68Å b: 119.98Å c: 73.54Å
α: 90° β: 98.91° γ: 90°
R R work R free
0.245 0.241 0.285
Expression systems:
  • Escherichia coli
  • Not provided