PDBe 2hwl

X-ray diffraction
2.4Å resolution

Crystal structure of thrombin in complex with fibrinogen gamma' peptide

Released:
Source organism: Homo sapiens

Function and Biology Details

Reaction catalysed:
Selective cleavage of Arg-|-Gly bonds in fibrinogen to form fibrin and release fibrinopeptides A and B.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Thrombin light chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 36 amino acids
Theoretical weight: 4.1 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P00734 (Residues: 328-363; Coverage: 6%)
Gene name: F2
Thrombin heavy chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 259 amino acids
Theoretical weight: 29.69 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P00734 (Residues: 364-622; Coverage: 43%)
Gene name: F2
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
Fibrinogen gamma chain Chain: P
Molecule details ›
Chain: P
Length: 14 amino acids
Theoretical weight: 1.8 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P02679 (Residues: 439-452; Coverage: 3%)
Gene names: FGG, PRO2061

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P21
Unit cell:
a: 66.397Å b: 81.523Å c: 70.256Å
α: 90° β: 109.35° γ: 90°
R-values:
R R work R free
0.214 0.214 0.249
Expression systems:
  • Cricetulus griseus
  • Not provided