› 2hf8

X-ray diffraction
2.1Å resolution

Crystal structure of HypB from Methanocaldococcus jannaschii in the triphosphate form, in complex with zinc

Released:
DOI: 10.2210/pdb2hf8/pdb
PDB entry 2hf8 coloured by chain, front view.
PDB entry 2hf8 coloured by chain, side view.
PDB entry 2hf8 coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii
Primary publication:
Structural insights into HypB, a GTP-binding protein that regulates metal binding.
Gasper R, Scrima A, Wittinghofer A
J Biol Chem 281 27492-502 (2006)
PMID: 16807243

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176495 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable hydrogenase maturation factor HypB Chains: A, B
Molecule details ›
Chains: A, B
Length: 226 amino acids
Theoretical weight: 24.99 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q57884 (Residues: 1-221; Coverage: 100%)
Gene names: MJ0442, hypB
Sequence domains: CobW/HypB/UreG, nucleotide-binding domain
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand ZN 4 x ZN
Ligand GSP 2 x GSP
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P212121
Unit cell:
a: 43.12Å b: 68.13Å c: 155.99Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.201 0.242
Expression system: Escherichia coli

Quick links

Citations

17 review citations

The septin family of GTPases: architecture and dynamics.
Weirich et al. (2008)
16 more

11 mentions without citation

Specific metal recognition in nickel trafficking.
Higgins et al. (2012)
10 more