PDBe 2h4j

X-ray diffraction
2.1Å resolution

Sir2-deacetylated peptide (from enzymatic turnover in crystal)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
NAD-dependent protein deacetylase Chain: A
Molecule details ›
Chain: A
Length: 246 amino acids
Theoretical weight: 27.57 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9WYW0 (Residues: 1-246; Coverage: 100%)
Gene names: TM_0490, cobB, sir2
Sequence domains: Sir2 family
Structure domains:
Cellular tumor antigen p53 Chain: D
Molecule details ›
Chain: D
Length: 18 amino acids
Theoretical weight: 2.1 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P04637 (Residues: 372-389; Coverage: 5%)
Gene names: P53, TP53

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P212121
Unit cell:
a: 46.049Å b: 59.683Å c: 107.35Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.198 0.246
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided