PDBe 2h1l

X-ray diffraction
3.16Å resolution

The Structure of the Oncoprotein SV40 Large T Antigen and p53 Tumor Suppressor Complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 12-mer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Large T antigen Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 370 amino acids
Theoretical weight: 42.65 KDa
Source organism: Macaca mulatta polyomavirus 1
Expression system: Escherichia coli
UniProt:
  • Canonical: P03070 (Residues: 260-627; Coverage: 52%)
Structure domains:
Cellular tumor antigen p53 Chains: M, N, O, P, Q, R, S, T, U, V, W, X
Molecule details ›
Chains: M, N, O, P, Q, R, S, T, U, V, W, X
Length: 203 amino acids
Theoretical weight: 22.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04637 (Residues: 92-292; Coverage: 51%)
Gene names: P53, TP53
Sequence domains: P53 DNA-binding domain
Structure domains: Immunoglobulin-like

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 127.019Å b: 182.718Å c: 262.061Å
α: 90° β: 92.08° γ: 90°
R-values:
R R work R free
0.069 0.261 0.308
Expression system: Escherichia coli