PDBe 2gs6

X-ray diffraction
2.6Å resolution

Crystal Structure of the active EGFR kinase domain in complex with an ATP analog-peptide conjugate

Released:

Function and Biology Details

Reaction catalysed:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Peptide Chain: B
Molecule details ›
Chain: B
Length: 13 amino acids
Theoretical weight: 1.54 KDa
Source organism: Synthetic construct
Expression system: Not provided
Epidermal growth factor receptor Chain: A
Molecule details ›
Chain: A
Length: 330 amino acids
Theoretical weight: 37.56 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P00533 (Residues: 696-1022; Coverage: 28%)
Gene names: EGFR, ERBB, ERBB1, HER1
Sequence domains: Protein tyrosine kinase
Structure domains:

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: I23
Unit cell:
a: 144.182Å b: 144.182Å c: 144.182Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.206 0.257
Expression systems:
  • Not provided
  • Spodoptera frugiperda