PDBe 2gs0

Solution NMR

NMR structure of the complex between the PH domain of the Tfb1 subunit from TFIIH and the activation domain of p53


Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
General transcription and DNA repair factor IIH subunit TFB1 Chain: A
Molecule details ›
Chain: A
Length: 115 amino acids
Theoretical weight: 12.9 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
  • Canonical: NEW P32776 (Residues: 2-115; Coverage: 18%)
Gene names: D9740.3, TFB1, YDR311W
Sequence domains: TFIIH p62 subunit, N-terminal domain
Structure domains: Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)
Cellular tumor antigen p53 Chain: B
Molecule details ›
Chain: B
Length: 54 amino acids
Theoretical weight: 6.02 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: NEW P04637 (Residues: 20-73; Coverage: 14%)
Gene names: P53, TP53
Sequence domains: Transactivation domain 2

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 87%
Refinement method: Simulated annealing, with a combination of torsion angle and Cartesian dynamics.
Chemical shifts: BMR6225  
Expression system: Escherichia coli