PDBe 2ghq

X-ray diffraction
2.05Å resolution

CTD-specific phosphatase Scp1 in complex with peptide C-terminal domain of RNA polymerase II

Released:

Function and Biology Details

Reactions catalysed:
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 181 amino acids
Theoretical weight: 20.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9GZU7 (Residues: 77-256; Coverage: 69%)
Gene names: CTDSP1, NIF3, NLIIF, SCP1
Sequence domains: NLI interacting factor-like phosphatase
Structure domains: HAD superfamily/HAD-like
DNA-directed RNA polymerase II subunit RPB1 Chains: C, D
Molecule details ›
Chains: C, D
Length: 9 amino acids
Theoretical weight: 1.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P24928 (Residues: 1795-1803; Coverage: 1%)
Gene names: POLR2, POLR2A

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: C2
Unit cell:
a: 126.067Å b: 78.813Å c: 62.992Å
α: 90° β: 112.17° γ: 90°
R-values:
R R work R free
0.23 0.218 0.248
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli