› 2gf3

X-ray diffraction
1.3Å resolution

Structure of the complex of monomeric sarcosine with its substrate analogue inhibitor 2-furoic acid at 1.3 A resolution.

Released:
DOI: 10.2210/pdb2gf3/pdb
PDB entry 2gf3 coloured by chain, front view.
PDB entry 2gf3 coloured by chain, side view.
PDB entry 2gf3 coloured by chain, top view.
Source organism: Bacillus sp. (in: Bacteria)
Entry authors: Chen Z, Trickey P, Jorns MS, Mathews FS

Function and Biology Details

Reaction catalysed: 
Sarcosine + H(2)O + O(2) = glycine + formaldehyde + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154199 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Monomeric sarcosine oxidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 389 amino acids
Theoretical weight: 43.1 KDa
Source organism: Bacillus sp. (in: Bacteria)
Expression system: Escherichia coli
UniProt:
  • Canonical: P40859 (Residues: 2-390; Coverage: 100%)
Gene names: sox, soxA
Sequence domains: FAD dependent oxidoreductase
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD
4 bound ligands:
Ligand CL 2 x CL
Ligand PO4 3 x PO4
Ligand FOA 4 x FOA
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 72.727Å b: 69.44Å c: 73.671Å
α: 90° β: 94.16° γ: 90°
R-values:
R R work R free
0.17 0.17 0.195
Expression system: Escherichia coli

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