PDBe 2gdz

X-ray diffraction
1.65Å resolution

Crystal structure of 15-hydroxyprostaglandin dehydrogenase type1, complexed with NAD+

Released:

Function and Biology Details

Reaction catalysed:
(5Z,13E,15S)-11-alpha,15-dihydroxy-9-oxoprost-5,13-dienoate + NAD(+) = (5Z,13E)-11-alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NADH
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
15-hydroxyprostaglandin dehydrogenase [NAD(+)] Chain: A
Molecule details ›
Chain: A
Length: 267 amino acids
Theoretical weight: 29.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P15428 (Residues: 3-256; Coverage: 96%)
  • Best match: P15428-3 (Residues: 1-135)
Gene names: HPGD, PGDH1, SDR36C1
Sequence domains: short chain dehydrogenase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NAD 1 x NAD
No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: P41212
Unit cell:
a: 49.54Å b: 49.54Å c: 195.771Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.176 0.212
Expression system: Escherichia coli