PDBe 2g54

X-ray diffraction
2.25Å resolution

Crystal structure of Zn-bound human insulin-degrading enzyme in complex with insulin B chain


Function and Biology Details

Reaction catalysed:
Degradation of insulin, glucagon and other polypeptides. No action on proteins.
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Insulin-degrading enzyme Chains: A, B
Molecule details ›
Chains: A, B
Length: 990 amino acids
Theoretical weight: 114.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: P14735 (Residues: 42-1019; Coverage: 96%)
  • Best match: P14735-2 (Residues: 1-464)
Gene name: IDE
Sequence domains:
Structure domains: Metalloenzyme, LuxS/M16 peptidase-like
Insulin B chain Chains: C, D
Molecule details ›
Chains: C, D
Length: 30 amino acids
Theoretical weight: 3.43 KDa
Source organism: Homo sapiens
Expression system: Not provided
  • Canonical: P01308 (Residues: 25-54; Coverage: 35%)
Gene name: INS

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P65
Unit cell:
a: 262.528Å b: 262.528Å c: 90.503Å
α: 90° β: 90° γ: 120°
R R work R free
0.206 0.206 0.233
Expression systems:
  • Escherichia coli
  • Not provided