PDBe 2g4d

X-ray diffraction
2.8Å resolution

Crystal structure of human SENP1 mutant (C603S) in complex with SUMO-1

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Sentrin-specific protease 1 Chains: A, C
Molecule details ›
Chains: A, C
Length: 205 amino acids
Theoretical weight: 24.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: NEW Q9P0U3 (Residues: 440-644; Coverage: 32%)
Gene name: SENP1
Sequence domains: Ulp1 protease family, C-terminal catalytic domain
Structure domains: Adenoviral Proteinase; Chain A
Small ubiquitin-related modifier 1 Chains: B, D
Molecule details ›
Chains: B, D
Length: 78 amino acids
Theoretical weight: 9.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: NEW P63165 (Residues: 20-97; Coverage: 77%)
Gene names: OK/SW-cl.43, SMT3C, SMT3H3, SUMO1, UBL1
Sequence domains: Ubiquitin-2 like Rad60 SUMO-like
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P43
Unit cell:
a: 97.308Å b: 97.308Å c: 102.58Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.248 0.248 0.278
Expression system: Escherichia coli BL21