PDBe 2g47

X-ray diffraction
2.1Å resolution

Crystal structure of human insulin-degrading enzyme in complex with amyloid-beta (1-40)

Released:

Function and Biology Details

Reaction catalysed:
Degradation of insulin, glucagon and other polypeptides. No action on proteins. 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Amyloid-beta protein 40 Chains: C, D
Molecule details ›
Chains: C, D
Length: 40 amino acids
Theoretical weight: 4.34 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P05067 (Residues: 672-711; Coverage: 5%)
Gene names: A4, AD1, APP
Insulin-degrading enzyme Chains: A, B
Molecule details ›
Chains: A, B
Length: 990 amino acids
Theoretical weight: 114.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P14735 (Residues: 42-1019; Coverage: 96%)
  • Best match: P14735-2 (Residues: 1-464)
Gene name: IDE
Sequence domains:
Structure domains: Cytochrome Bc1 Complex; Chain A, domain 1

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P65
Unit cell:
a: 262.433Å b: 262.433Å c: 90.706Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.203 0.203 0.223
Expression systems:
  • Not provided
  • Escherichia coli