› 2fwr

X-ray diffraction
2.6Å resolution

Structure of Archaeoglobus Fulgidis XPB

Released:
DOI: 10.2210/pdb2fwr/pdb
PDB entry 2fwr coloured by chain, front view.
PDB entry 2fwr coloured by chain, side view.
PDB entry 2fwr coloured by chain, top view.
Source organism: Archaeoglobus fulgidus
Primary publication:
Conserved XPB core structure and motifs for DNA unwinding: implications for pathway selection of transcription or excision repair.
Fan L, Arvai AS, Cooper PK, Iwai S, Hanaoka F, Tainer JA
Mol Cell 22 27-37 (2006)
PMID: 16600867

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128186 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA 3'-5' translocase XPB Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 472 amino acids
Theoretical weight: 54.02 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O29889 (Residues: 1-452; Coverage: 100%)
Gene names: AF_0358, xpb
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand PO4 8 x PO4
Ligand IPA 3 x IPA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P1
Unit cell:
a: 49.085Å b: 97.963Å c: 113.731Å
α: 79.03° β: 85.54° γ: 89.69°
R-values:
R R work R free
0.221 0.237 0.3
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

29 review citations

Nucleotide excision repair in eukaryotes.
Schärer OD. (2013)
28 more

14 mentions without citation

XPB and XPD helicases in TFIIH orchestrate DNA duplex opening and damage verification to coordinate repair with transcription and cell cycle via CAK kinase.
Fuss et al. (2011)
13 more