PDBe 2fr1

X-ray diffraction
1.79Å resolution

The first ketoreductase of the erythromycin synthase (crystal form 2)


Function and Biology Details

Reaction catalysed:
Propanoyl-CoA + 6 (2S)-methylmalonyl-CoA + 6 NADPH = 6-deoxyerythronolide B + 7 CoA + 6 CO(2) + H(2)O + 6 NADP(+)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
6-deoxyerythronolide-B synthase EryA1, modules 1 and 2 Chain: A
Molecule details ›
Chain: A
Length: 486 amino acids
Theoretical weight: 51.08 KDa
Source organism: Saccharopolyspora erythraea
Expression system: Escherichia coli BL21(DE3)
  • Canonical: Q03131 (Residues: 1391-1872; Coverage: 14%)
Gene name: eryA
Sequence domains:

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P1
Unit cell:
a: 42.043Å b: 42.939Å c: 61.044Å
α: 90.03° β: 103.81° γ: 100.71°
R R work R free
0.227 0.227 0.257
Expression system: Escherichia coli BL21(DE3)