PDBe 2fp4

X-ray diffraction
2.08Å resolution

Crystal structure of pig GTP-specific succinyl-CoA synthetase in complex with GTP

Released:
Source organism: Sus scrofa

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Succinate--CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 305 amino acids
Theoretical weight: 32.17 KDa
Source organism: Sus scrofa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O19069 (Residues: 43-346; Coverage: 88%)
Gene name: SUCLG1
Sequence domains:
Structure domains:
Succinate--CoA ligase [GDP-forming] subunit beta, mitochondrial Chain: B
Molecule details ›
Chain: B
Length: 395 amino acids
Theoretical weight: 42.64 KDa
Source organism: Sus scrofa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P53590 (Residues: 39-433; Coverage: 91%)
Gene name: SUCLG2
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P65
Unit cell:
a: 132.61Å b: 132.61Å c: 72.53Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.214 0.195 0.242
Expression system: Escherichia coli BL21(DE3)