PDBe 2fju

X-ray diffraction
2.2Å resolution

Activated Rac1 bound to its effector phospholipase C beta 2

Released:
Source organism: Homo sapiens
Primary publication:
Crystal structure of Rac1 bound to its effector phospholipase C-beta2.
Nat. Struct. Mol. Biol. 13 1135-40 (2006)
PMID: 17115053

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ras-related C3 botulinum toxin substrate 1 Chain: A
Molecule details ›
Chain: A
Length: 178 amino acids
Theoretical weight: 19.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P63000 (Residues: 1-177; Coverage: 92%)
Gene names: MIG5, RAC1, TC25
Structure domains: P-loop containing nucleotide triphosphate hydrolases
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 Chain: B
Molecule details ›
Chain: B
Length: 799 amino acids
Theoretical weight: 91.03 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q00722 (Residues: 1-803; Coverage: 67%)
  • Best match: Q00722-2 (Residues: 1-799)
Gene name: PLCB2
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P3221
Unit cell:
a: 185.822Å b: 185.822Å c: 93.823Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.208 0.208 0.226
Expression systems:
  • Escherichia coli
  • Spodoptera frugiperda