PDBe 2fhj

X-ray diffraction
2Å resolution

Crystal structure of formylmethanofuran: tetrahydromethanopterin formyltransferase in complex with its coenzymes

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Formylmethanofuran--tetrahydromethanopterin formyltransferase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 296 amino acids
Theoretical weight: 31.69 KDa
Source organism: Methanopyrus kandleri
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q49610 (Residues: 1-296; Coverage: 100%)
Gene names: MK0116, ftr
Sequence domains:
Structure domains: Alpha-Beta Plaits
Formylmethanofuran--tetrahydromethanopterin formyltransferase Chain: D
Molecule details ›
Chain: D
Length: 296 amino acids
Theoretical weight: 31.7 KDa
Source organism: Methanopyrus kandleri
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q49610 (Residues: 1-296; Coverage: 100%)
Gene names: MK0116, ftr
Sequence domains:
Structure domains: Alpha-Beta Plaits

Ligands and Environments


1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21
Unit cell:
a: 85Å b: 74.15Å c: 103.87Å
α: 90° β: 113.54° γ: 90°
R-values:
R R work R free
0.221 0.219 0.256
Expression system: Escherichia coli BL21(DE3)