PDBe 2esv

X-ray diffraction
2.6Å resolution

Structure of the HLA-E-VMAPRTLIL/KK50.4 TCR complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Soluble HLA class I histocompatibility antigen, alpha chain E Chain: A
Molecule details ›
Chain: A
Length: 275 amino acids
Theoretical weight: 31.87 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: NEW P13747 (Residues: 23-297; Coverage: 82%)
Gene names: HLA-6.2, HLA-E, HLAE
Sequence domains: Immunoglobulin C1-set domain
Structure domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: NEW P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Protein UL40 Chain: P
Molecule details ›
Chain: P
Length: 9 amino acids
Theoretical weight: 1.01 KDa
Source organism: Human herpesvirus 5 strain AD169
Expression system: Not provided
UniProt:
  • Canonical: NEW P16780 (Residues: 15-23; Coverage: 4%)
Gene name: UL40
T cell receptor alpha constant Chain: D
Molecule details ›
Chain: D
Length: 199 amino acids
Theoretical weight: 22.22 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: NEW P01848 (Residues: 1-89; Coverage: 64%)
Gene names: TCRA, TRAC
Structure domains: Immunoglobulins
T cell receptor beta constant 1 Chain: E
Molecule details ›
Chain: E
Length: 240 amino acids
Theoretical weight: 27.28 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: NEW P01850 (Residues: 1-129; Coverage: 73%)
Gene name: TRBC1
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: C2
Unit cell:
a: 235.096Å b: 41.462Å c: 112.471Å
α: 90° β: 114.66° γ: 90°
R-values:
R R work R free
0.218 0.214 0.292
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided