PDBe 2eb1

X-ray diffraction
2Å resolution

Crystal Structure of the C-Terminal RNase III Domain of Human Dicer

Released:

Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage to 5'-phosphomonoester.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endoribonuclease Dicer Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 200 amino acids
Theoretical weight: 23.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UPY3 (Residues: 1659-1852; Coverage: 10%)
  • Best match: Q9UPY3-2 (Residues: 1659-1788)
Gene names: DICER, DICER1, HERNA, KIAA0928
Sequence domains: Ribonuclease III domain
Structure domains: Ribonuclease III domain

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: C2221
Unit cell:
a: 88.602Å b: 199.743Å c: 119.64Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.211 0.234
Expression system: Escherichia coli