› 2e28

X-ray diffraction
2.4Å resolution

Crystal structure analysis of pyruvate kinase from Bacillus stearothermophilus

Released:
DOI: 10.2210/pdb2e28/pdb
PDB entry 2e28 coloured by chain, front view.
PDB entry 2e28 coloured by chain, side view.
PDB entry 2e28 coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
Crystal structure of pyruvate kinase from Geobacillus stearothermophilus.
Suzuki K, Ito S, Shimizu-Ibuka A, Sakai H
J Biochem 144 305-12 (2008)
PMID: 18511452

Function and Biology Details

Reaction catalysed: 
ATP + pyruvate = ADP + phosphoenolpyruvate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169723 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyruvate kinase Chain: A
Molecule details ›
Chain: A
Length: 587 amino acids
Theoretical weight: 62.36 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q02499 (Residues: 1-587; Coverage: 100%)
Gene name: pyk
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P6222
Unit cell:
a: 145.97Å b: 145.97Å c: 118.03Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.226 0.226 0.294
Expression system: Escherichia coli

Quick links

Citations

2 review citations

An overview of structure, function, and regulation of pyruvate kinases.
Schormann et al. (2019)
1 more

9 mentions without citation

The Blueprint of a Minimal Cell: MiniBacillus.
Reuß et al. (2016)
8 more