PDBe 2dws

X-ray diffraction
1.85Å resolution

Cu-containing nitrite reductase at pH 8.4 with bound nitrite


Function and Biology Details

Reaction catalysed:
Nitric oxide + H(2)O + ferricytochrome c = nitrite + ferrocytochrome c + 2 H(+)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Copper-containing nitrite reductase Chain: A
Molecule details ›
Chain: A
Length: 328 amino acids
Theoretical weight: 35.8 KDa
Source organism: Rhodobacter sphaeroides
Expression system: Escherichia coli BL21(DE3)
  • Canonical: Q53239 (Residues: 44-371; Coverage: 96%)
Gene names: Rsph17025_1595, nirK
Sequence domains:
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I711
Spacegroup: R3
Unit cell:
a: 82.204Å b: 82.204Å c: 146.152Å
α: 90° β: 90° γ: 120°
R R work R free
0.168 0.168 0.199
Expression system: Escherichia coli BL21(DE3)