PDBe 2dwp

X-ray diffraction
2.7Å resolution

A pseudo substrate complex of 6-phosphofructo-2-kinase of PFKFB

Released:

Function and Biology Details

Reactions catalysed:
ATP + D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate
Beta-D-fructose 2,6-bisphosphate + H(2)O = D-fructose 6-phosphate + phosphate
Biochemical function:
Cellular component:

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 Chain: A
Molecule details ›
Chain: A
Length: 520 amino acids
Theoretical weight: 59.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q16875 (Residues: 1-520; Coverage: 100%)
Gene name: PFKFB3
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CAMD BEAMLINE GCPCC
Spacegroup: P6522
Unit cell:
a: 101.99Å b: 101.99Å c: 258.07Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.251 0.224 0.264
Expression system: Escherichia coli